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Contact SupplierFish acute toxicity tests are used to assess potential risk to fish species and for other ecological regulatory needs associated with surface water contaminants. To meet EPA data requirements the test is typically conducted in three different fish species: a cold-water freshwater species, a warm-water freshwater species, and a marine/estuarine species Workgroup is evaluating ecotoxicology methods involving animal testing and what alternatives exist to replace, reduce, or refine animal use in the acute fish toxicity assay. NICEATM is also working with EPA and other partners to explore reducing animal use for fish acute toxicity testing.