Shenzhen Biokau Sci-tech Co.,ltd

Shenzhen, China

• MOQ 1 Piece(s)

We are offering trichothecenes elisa test kit, 1. General t-2 toxin is a mycotoxin produced by various fusarium. It mainly pollutes wheat, barley, corn and other food crops and their products, which constitute a great hazard to human health and animal husbandry. T2 mainly affects the function of the blood, liver, kidney, pancreatic muscle and lymphocyte. The clinical symptoms of t2 toxin poisoning is anorexia, vomiting, diarrhea, growth retardation, reproductive and neurological dysfunction etc.the use of trichothecenes(t2) elisa test kit to analyze t2 residue of samples is fast and accurate. This kit is a new generation of fungal toxins detection products in application of elisa technology and the operation time is shorter than 60min, to minimize operating errors and work intensity. 7.2 preparation of wheat sample 7.2 preparation of wheat sample ã�â¢ã¯â¿â½ã¯â¿â½ weigh 1g of ground sample into a suitable container and add 20ml of sample extract solution ã�â¢ã¯â¿â½ã¯â¿â½ shake vigorously for 5 minutes (manually or with shaker) ã�â¢ã¯â¿â½ã¯â¿â½ filter the extract through filter ã�â¢ã¯â¿â½ã¯â¿â½ take 1ml supernatant, add 2ml methylene dichloride, shake, centrifuge for 5min at 4000rmin, take methylene dichloride layer, blow to dryness at 50ã�â¢ã¯â¿â½ã¯â¿â½. Ã�â¢ã¯â¿â½ã¯â¿â½ add 5 ml 10% methanol, shake evenly. Ã�â¢ã¯â¿â½ã¯â¿â½ take the liquid to test dilution factor: 100

• MOQ 1 Carton

Offers zearalenone elisa test kit. 1. General zearalenone are commonly found in corn and animal feed. 2. Test principle this test kit is based on the competitive enzyme immunoassay for the detection of zearalenone in the sample. The coupling antigens are pre-coated on the micro-well stripes. The zearalenone in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-zearalenone antibodies. After the addition of the enzyme conjugate, the tmb substrate is added for coloration. The optical density (od) value of the sample has a negative correlation with the zearalenone in it. This value is compared to the standard curve and the zearalenone concentration is subsequently obtained. 3. Application this test kit can be used to detect zearalenone in corn and feed samples qualitatively and quantitatively . 4. Reagents provided each kit contains sufficient materials for 96 measurements (including standard analyses). Each test kit contains: 1 x microtiter plate (12 strips with 8 removable wells each) coated with capture antigen 6 x concentrated standards, 1 ml each: 0 ppb (zero standard), 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb 1 x enzyme conjugate.............................................................6 ml 1 x antibody working solution.............................................................................6 ml 1 x substrate a................................................................ ...................................6 ml 1 x substrate b............................................................................................ ......6 ml 1 x stop solution..................................................................................................6 ml 1 x concentrated washing buffer(10x)................................................................40ml 5. Reagents required but not provided.

• MOQ 1 Carton

Offers total aflatoxins elisa test kit. 1. General aflatoxins are secondary metabolites of the fungi species aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed. 2. Test principle this test kit is based on the competitive enzyme immunoassay for the detection of aflatoxins in the sample. The coupling antigens are pre-coated on the micro-well stripes. The aflatoxins in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-aflatoxins antibodies. After the addition of the enzyme conjugate, the tmb substrate is added for coloration. The optical density (od) value of the sample has a negative correlation with the aflatoxins in it. This value is compared to the standard curve and the aflatoxins concentration is subsequently obtained. 3. Application this test kit can be used to detect total aflatoxins in maize, corn, feed, edible oil and other samples qualitatively and quantitatively. 4. Reagents provided each kit contains sufficient materials for 96 measurements (including standard analyses). Each test kit contains: 1 x microtiter plate (12 strips with 8 removable wells each) coated with capture antigen 6 x standards, 1 ml each: 0 ppb (zero standard), 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb 1 x high concentration standard, 1ml 100ppb 1 x enzyme conjugate.............................................. 10 ml 1 x antibody working solution.............................................6 ml 1 x substrate a.........................................................................10ml 1 x substrate b............................................................ ......10ml 1 x stop solution......................................................................6ml 1 x concentrated washing buffer.......................................40ml.

• MOQ 1 Set(s)

We are offering deoxynivalenol elisa test kit. 1. general deoxynivalenol, also known as vomitoxin belongs to the trichothecene mold streptozotocin family, produced by some fusarium fungi. Deoxynivalenol are presented in wheat, barley, corn and other grain seeds, the content is usually mg kg (μg ml, ppm) level. Because of their high cytotoxic and immunosuppressive properties, deoxynivalenol constitutes a health threat to human and animal. This kit is a new generation of fungal toxins detection products in application of elisa technology, to minimize operating errors and work intensity.

• MOQ 1 Set(s)

We offer fumonisin b1 elisa kit. 1. Principle this test kit is based on the competitive enzyme immunoassay for the detection of fumonisin b1 in the sample. The coupling antigen is pre-coated on the micro-well stripes. The fumonisin b1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-fumonisin b1 antibodies. After the addition of the enzyme conjugate, the tmb substrate is added for coloration. The optical density (od) value of the testing sample has a negative correlation with the fumonisin b1 concentration in the sample. This value is compared to the standard curve and the fumonisin b1 concentration is subsequently obtained. 2. Technical specifications sensitivity : 10 ppb detection limit: grain, corn, feed......................................................................... 0.5 ppm linear range: 10ngml~200ngml recovery rate: grain, corn, feed........................................................................... 90±15% precision: intra-assay cv

• MOQ 1 Set(s)

We offer ochratoxin a elisa test kit. 1. general ochratoxin a are secondary metabolites of aspergillus and penicillium strains, exist widely in grain and grain products, spices and coffee beans and other products. Ochratoxin a has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic effects, which is serious harmful to human health. Thin-layer chromatography (tlc) and high-performance liquid chromatography (hplc) has been a common method of ochratoxin a detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of ochratoxin a elisa kit to analyze ochratoxin a residue of samples is fast and accurate. This kit is a new generation of fungal toxins detection products in application of elisa technology, to minimize operating errors and work intensity. 2. principle the test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with ochratoxin antigen. Free ochratoxin in sample solutions and the immobilized antigen compete for ochratoxin enzyme conjugate. While ochratoxin antibody and hrp secondary antibody (hrp material) combined, via the tmb substrate show color, sample absorbance values ??of ochratoxin has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw ochratoxin content in samples. 3. application this test kit can be used to detect ochratoxin in corn and corn products, soybeans, oats and other crops and samples qualitatively and quantitatively. 4. technical specifications sensitivity: 0.1 ppb detection limit: corn, soybean, whole wheat etc. …………………………………………………1ppb ……………………………………………………………………………….0.1ppb recovery rate:

• MOQ 1 Set(s)

We offer aflatoxin m1 afm1 elisa test kit. 1. general aflatoxins are secondary metabolites of the fungi species aspergillus flavus, parasiticus and nomius. aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed. Aflatoxin m1 is the hydroxylated metabolite of aflatoxin b1, also a strong carcinogen. Milk and its products are one of the food susceptible to aflatoxin m1 contamination. The methods to test aflatoxin m1 residue is high-performance liquid chromatography (hplc), thin layer chromatography (tlc) etc. The use of aflatoxin m1 elisa kit to analyze aflatoxin m1 residue of samples is fast and accurate. This kit is a new generation of fungal toxins detection products in application of elisa technology and the operation time is shorter than 60min, to minimize operating errors and work intensity. 2. principle the test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with aflatoxin m1 antigen. Free aflatoxin m1 in sample solutions and the immobilized antigen compete for aflatoxin enzyme conjugate. While aflatoxin m1 antibody and hrp secondary antibody (hrp material) combined, via the tmb substrate show color, sample absorbance values ??of aflatoxin m1 has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw aflatoxin m1 content in samples. 3. application this test kit can be used to detect aflatoxin m1 in liquid milk, fermented milk, milk powder, cream, condensed milk, cheese etc. Samples qualitatively and quantitatively. 4. technical specifications sensitivity: 0.05 ppb

• MOQ 1 Set(s)

We offer aflatoxins b1 (afb1) elisa test kit. 1. general aflatoxins are secondary metabolites of the fungi species aspergillus flavus, parasiticus and nomius. Aflatoxins belong to the strongest natural occurring carcinogenic substances, commonly found in corn, peanuts, cotton seed, human blood and animal feed.aflatoxin b1 appears nearly in all cases together with aflatoxin b2, g1 and g2 and it is the analyte with the highest toxic significance.thin-layer chromatography has been a common method of aflatoxin detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of aflatoxin b1 elisa kit to analyze aflatoxin b1 residue of samples is fast and accurate. Refer to the national standard gb t 5009.22-2003. This kit is a new generation of fungal toxins detection products in application of elisa technology and the operation time is shorter than 60min, to minimize operating errors and work intensity. 2. principle the test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with aflatoxin antigen. Free aflatoxin in sample solutions and the immobilized antigen compete for aflatoxin enzyme conjugate. Any unbound enzyme conjugate is then removed in a washing step. Substratechromogen is added to the wells, bound enzyme conjugate converts the chromogen into a blue product. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorbance is inversely proportional to the aflatoxin concentration in the sample. 3. application this test kit can be used to detect aflatoxin b1 in maize, rice, wheat, beans, peanuts, peanut butter, feed, edible oil and other samples qualitatively and quantitatively. 4. technical specifications sensitivity: 0.1 ppb detection limit:

We are offering bovine tuberculosis antibody rapid test card, 1. Principle this test strip is based on fast immuno-chromatography technique to detect tuberculosis ab in bovine serum and plasma qualitatively. When testing, tuberculosis ab in sample combine with antigen coated by colloid gold forming complexes, moving forward to the other head. When reaching the t-line, the specific antigen on the membrane capture the complexes and appear a t line, c line appears, means the test is valid. The t line appears, means there is tuberculosis ab in the sample. 2. Specifications: 25 testskit 3. Components 1) test cassette 25 pieces 2) dropper 25 pieces 3) manual 1 piece 4. Operation procedures 1) read the manual carefully and return the test card and sample to room temperature before use. 2) take out test card, use dropper to absorb serum sample, drop 3 drops into well mark “s

• MOQ 1 Piece(s)

We are offering canine toxoplasma gondii antibody rapid test card, ø principle this rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the toxoplasma antibody in serum. The whole test only need 20 mins. Easy to operate, fast, accurate, high sensitivity, intuitive result, easy to judge. This test using double antigen sandwich method, can detect specify toxoplasma antibody in animal serum of cats, dogs, pigs, rabbits, mice etc ø test procedure 1) add 2 drops (100 ul) serum sample into the well on the test card; 2) place the test card on the desktop flatly, incubate for 5-20 minute, then judge the result. the result of more than 20 mins can only be used as a reference. Ø 1) positive: control line and test line are both seen red, the more the antibody exist, the thicker the color appear; 2) weak positive: control line and test line are both seen red, but the color of test line is very light; 3) negative: only control line is seen red; 4) invalidation: control line isn’t seen red. Ø notice use the test card as soon as possible once open the aluminum foil, or the test card will be invalidated, store at normal temperature and the shelf life is 18 months. Ø storage store at 2～30℃ in sealed, dry environment.

• MOQ 1 Piece(s)

We are offering beta lactamase rapid test strip, 1. Brief this product is used for testing î²-lactamase residue in raw milk. Sensitivity is as following table 1, suitable for all kinds of enterprises and inspection agency. table 1: relation between incubation time and sensitivity.

• MOQ 1 Piece(s)

We are offering nitrofuran rapid test strip. 1. Brief this product is used for testing nitrofuran(aoz) residue in honey samples. The whole testing needs about 2h, suitable for all kinds of enterprises and inspection agencies. The detection limit is 0.5ppb（ugkg） 2. Components 1) nitrofuran (aoz) colloidal gold rapid test strip (including tablet, gold label well, dropper, desiccant) 50 pieceskit 2) manuals 1 piecekit 3) 2-nitrobenzaldehyde (c7h5no3) 1 bottlekit 4) nitrofuran redissolving solution 1 bottlekit

• MOQ 1 Piece(s)

We are offering nitrofuran rapid test strip. 1. Brief this product is used for testing nitrofuran(amoz) residue in honey samples. The whole testing needs about 2h, suitable for all kinds of enterprises and inspection agencies. The detection limit is 0.5ppb�ugkg� 2. Components 1) nitrofuran (amoz) colloidal gold rapid test strip (including tablet, gold label well, dropper, desiccant) 50 pieceskit 2) manuals 1 piecekit 3) 2-nitrobenzaldehyde (c7h5no3) 1 bottlekit 4) nitrofuran redissolving solution 1 bottlekit 3. Equipments required but not provided micropipettes, nitrogen-drying device or air-drying device, water bath, centrifuge, balance (a reciprocal sensibility of 0.01 g); 4. Sample preparation] 1) weigh 3g honey sample into 50ml centrifuge tube, add 4ml deionized water, 0.5ml 1m hcl, 0.2ml 2-nitrobenzaldehyde (c7h5no3), mix thoroughly for 3 min; 2) incubate in water bath at 60� for 1h; 3) take the tube out from the water bath, add 5ml 0.1m k2hpo4, 0.4ml 1m naoh, 6ml ethyl acetate, mix thoroughly for 1min, centrifuge at 4000rmin for 5min; 4) take 3ml supernatant into 5ml centrifuge tube, blow to dry by air at 60�; 5) add 0.5ml n-hexane and 0.3ml nitrofuran redissolving solution to the dry tube, mix evenly, centrifuge at 4000rmin for 1min (or put aside until stratification); 6) use micropipettes to absorb 100ul down-layer solution into gold label well, ready to test.

• MOQ 1 Piece(s)

Outbreaks of white spot syndrome (wss), causing serious mortality to cultured shrimp, have been occurring in the east asia since 1992. Wss has spread widely, and caused serious damage to shrimp farms in the world. Detection of white spot syndrome virus (wssv) in shrimps is very important for management of shrimp farms. Shrimp virus detection kit is a management tool based on immunoassay for shrimp aquaculture that can be used to assess the level of wssv infection in larva and shrimp during breeding. It is a lateral flow, one step , fast and easy. The results are read visually without any instruments. The test system employs unique monoclonal antibodies to selectively identify wssv in shrimps with a high degree of sensitivity. Kit contents each kit consists of following parts. test device microtube with 0.6ml buffer liquid (20mm, tbs) stick dropper specimen collection shrimps that look sick or unhealthy should be used. No special pre-treatment is necessary. The specimen collection may be stored up to 2 days at refrigerated condition (2-8 ¢ã¯â¿â½ã¯â¿â½). If it is necessary to store the specimen more than 3 days, it should be stored under frozen condition(-20ã�â¢ã¯â¿â½ã¯â¿â½ or below)

• MOQ 1 Piece(s)

We offers classical swine fever virus antigen rapid test strip. Principle this rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect classical swine fever virus antigen in whole blood, serum and plasma. Test procedure 1. Add 2 drops serum or whole blood sample into the well on the test card; 2. Place the test card on the desktop flatly, incubate for 20 minutes, then judge the result. Results judgement 1. Positive: control line and test line are both seen red, the more the antibody exist, the thicker the color appear; 2. Negative: only control line is seen red; 3. Invalidation: control line isn’t seen red. Notice 1. Use the test card as soon as possible once open the aluminum foil, or the test card will be invalidated, store at normal temperature and the shelf life is 18 months. 2. Whole blood sample may move slowly, add 1 drop pbs solution to promote sample moving in 1 minute from adding sample if necessary. Storage store at 2-30℃ hermetic dry environment.