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Rhodamine is a bright fluorescent red dye originally developed for water tracing applications. Rhodamine has subsequently been used in coloring automotive coolants (anti-freeze) and in inks. As a water tracing dye, Rhodamine has the ability to stain organic materials from plants, silt and dirt. With a state of the art fluorometer, detection of the dye is 10 ppt (parts per trillion) in ideal conditions and at 0.1 ppb (parts per billion) in polluted waters. It can also be detectable by the naked eye at higher concentrations. Rhodamine is a synthetic red to pink dye having brilliant fluorescent qualities with molecular formula C29H29N2O5ClNa2 and CAS Number: 37299-86-8. It is also known as Acid Red #388. Further, It is often used as a tracer within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be measured easily and inexpensively with instruments called fluorometers. Rhodamine dyes are soluble in water, methanol, and ethanol. Applications Biological Application In biological studies, rhodamine, was used to evaluate the bovine brain microvessel endothelial cell monolayers(fig1). Moreover, rhodamine is widely used to quantify P-glycoprotein (P-GP) functional efflux activity in vitro. And determination of human IgG by solid substrate room temperature phosphorescence immunoassay based on an antibody labeled with nanoparticles containing Rhodamine luminescent molecules. Medicinal Application Rhodamine has another application. For example, the determination of rhodamine by sequential injection technique for pharmacokinetic studies in the rat placenta, the sequential injection (SIA) technique was applied in pharmacokinetic studies of the transporter-mediated passage of a model substrate, rhodamine, through the dually perfused rat term placenta. Rhodamine: Handling and Storage Prior to working with Rhodamine you should be trained on its proper handling and storage. Precautions Keep away from heat. Keep away from sources of ignition. Empty containers pose a fire risk, evaporate the residue under a fume hood. Ground all equipment containing material. Do not ingest. Do not breathe dust. Wear suitable protective clothing In case of insufficient ventilation, wear suitable respiratory equipment If ingested, seek medical advice immediately and show the container or the label. Avoid contact with skin and eyes. Storage Keep container dry. Keep in a cool place. Ground all equipment containing material. Keep container tightly closed. Keep in a cool, w ell-ventilated place. Combustible materials should be stored away from extreme heat and away from strong oxidizing. agents. Rhodamine: Safety & Environment Safety & Environment is the first requirement. Since Rhodamine is classed as an 'irritant', it is necessary to observe certain elementary precautions. These include: Don’t place the skin in prolonged contact with Rhodamine or any of its solutions. Use water, preferably lukewarm, to thoroughly rinse any part of the body affected by this product. Wear gloves made from rubber or plastic. Avoid any inhalation of Rhodamine. Wear protective glasses when handling Rhodamine. Avoid working with equipment made from leather.