Douglas Scientific

• Manufacturer Suggested Volume Range0.2 - 10 µL / 25.0 µL (dependent on tip vol.
• MechanismSimultaneous air displacement
• Manufacturer PrecisionCV < 2%, at 1.0 µL

Expansion Block Module Dehydrator The Dehydrator, mounted in the Expansion Block Module, is used to dry down sample as Array Tape is passed and paused underneath. This attachment takes incoming dry air and heats it to a preset temperature. Dry down times vary based on the volume of sample present.

• Size335mm

Nexar Plate Stacker Nexar Plate Stacker The Plate Stacker, mounted in the Expansion Block Module, is used to store source or reagent plates up to 335mm high in each of the two removable extruded aluminum chutes. Each chute can be designed as an incoming or outgoing rack. Plates are automatically moved to and from the chutes without manual intervention through use of solenoids, a motorized lift arm, and conveyor system.

Soellex® High Throughput PCR Thermal Cycler Thermal Cycle 230, 400 Data Points in a Single Process Run The Soellex is an ultra high throughput end-point polymerase chain reaction (PCR) thermal cycler leveraging a 3-chamber water bath. The heating system in the Soellex tightly controls the temperature throughout the water bath delivering efficient and rapid energy transfer from the water, through the Array Tape® and to your samples. Thermal Cycle 230, 400 Data Points Simultaneously The Soellex is capable of simultaneously processing up to three spools of Array Tape, each with two hundred 384-well arrays per spool. Therefore, the Soellex has the potential to thermal cycle between 76, 800 and 230, 400 reaction wells in a single process run. Intuitive Thermal Cycler User Interface The touch screen user interface is simple, yet feature rich, providing the control necessary to process requirements. It includes: Intuitive run progress and monitoring feedback screen Thermal cycling protocol programming wizard Customizable alarms and warnings Remote computer monitoringnotification Reports generation and log file reviewer And more! High Throughput Energy Savings High throughput processing of up to 230, 400 samples in Array Tape requires one Soellex water bath - replacing the 18 water baths (32 plate capacity) or 150 block heaters required for microplates. These energy and space saving benefits put high throughput laboratories well on their way to achieving significantly greener, more environmentally sound operations. Common End Point PCR Chemistries The Soellex supports all common end-point PCR chemistries including but not limited to TaqMan™ and InvaderPLUS™. Versatile Microplate System Design The Soellex system design is versatile and supports PCR processing in traditional microtiter plates as well as in Array Tape. The Soellex can process up to 152 microplates (384-well, 11 mm tall) in a single run. Decrease Thermal Cycling Times Array Tape allows miniaturization of the reaction, has thin walls and low thermal mass (7 times less than a microplate) enabling rapid temperature changes contributing to shorter cycle times and improved results. Laboratory scientists who have experienced nonspecific binding issues recognize sharp temperature changes in Array Tape as a key advantage.

There is a demand for rapid and accurate nucleic acid analysis at the point of sample collection to enable professionals to quickly and accurately render a decision and take action. The AmpliFire is a portable point of use instrument optimized for isothermal chemistry with the capability to process eight samples simultaneously. With data storage, touch screen interface and easy analysis, the AmpliFire allows you to bring your lab anywhere! Features Up to eight samples can be processed simultaneously Touch screen user interface Portability enabled by rechargeable battery AmpliCode™ - a QR Code generator available with the instrument software, allows you to easily configure the instrument based on specific chemistries. Detection and Amplification Heating block supports up to eight samples per run Integrated detection system Multiple fluorescent channels monitored in real time Optimized for Isothermal Chemistry The AmpliFire allows true isothermal reactions at a single incubation temperature in reaction volumes as low as 20 µL. With the ability to use crude sample prep and the elimination of mid-run enzyme or buffer additions the AmpliFire truly is a portable, easy to use solution. Data Analysis With easy to use touch screen protocol generation and sample assignment you can be sure your data generation is simple. Real time data display with end-point calls allows you to view reactions as they occur. Portability of the instrument is enhanced with the ability to analyze and store up to 100 runs (800 samples) or share data via USB.

• THIN(0.3 mm)

Array Tape® Advanced Consumable Continuous Polymer Array Tape Array Tape is a continuous polymer strip, serially embossed with reaction wells in customized volumes and formats including 96-, 384- and 768-well arrays. Array Tape is thin (0.3 mm) and flexible, which allows up to 800 microplate equivalents to be spooled onto a single reel. It is a flexible microplate replacement for applications that enables a streamlined lab process. Reduced Cost Sub-Microliter Reactions Current applications support sub-microliter reactions (≤ 800 nL). These fluid volumes are significantly less than microtiter plates where the minimum practical reagent volume is approximately 5µL in a 384-well plate. The smaller reaction volumes in Array Tape reduce reagent costs by 80-90% compared to microplate-based systems. Array Tape not only enables significant increases in throughput due to inline processing, but also dramatically reduces cost per data point. Douglas Scientific provides clean consumable Array Tape - certified by an outside lab to be RNAse, DNAse, and pyrogen free.

Nexar® and Araya® User Interface Efficient Design and User Experience The Nexar and Araya® are designed to provide a simple and efficient user experience. The user interface (UI) provides flexibility in each key area: Nexar® User Interface System Configuration – The UI displays only the necessary options based on the system configuration. Auto or Manual - Systems can be operated in automatic mode with little to no user interaction or manually with full user control. For information on protocol generation, data management and scoring, view the Intellics® Software overview.

The Array Counter provides a quick way to count the number of arrays when specific run sizes are required. It can also be used to prepare Array Tape® for the centrifuge by winding it onto another reel to achieve the desired well direction (wells facing out).

Higher throughput in a smaller footprint! Related Downloads Hydrocycler2 Hydrocycler2 - How does it work Combining throughput, efficiency and robust design, the Hydrocycler2 offers an attractive alternative to traditional Peltier-based thermal cyclers for high-throughput PCR. Water conducts heat - Using water to conduct heat to a PCR sample offers multiple advantages including the ability to process many plates or arrays in parallel for high throughput with minimal variation in temperature from plate to plate or array to array. Fast cycling time - In addition, cycling time is 40% faster since there is no need to ramp temperatures up or down during the PCR cycle. Precise temperature control - Plates and arrays automatically move from one temperature bath to another and the temperature of each bath can be precisely controlled. Hydrocycler Why choose the Hydrocycler2? Increased Throughput Ability to process more than 145, 000 samples in an eight-hour day* Suitable for 96-, 384- and 1536-well plate formats and 384-well Nexar® or IntelliQube® Array Tape® with minimal system set up * Throughput is highly dependent on specific cycling conditions and chemistry selection. This estimate is based on 1536-plates and a standard KASP 36-cycle run totaling 1 hour 10 minutes. Efficient Amplification Circulation Jet ensures temperature uniformity in each tank for high PCR consistency and eliminates edge effect Multi-bath system delivers precise temperature control, enhances cycling time, and eliminates temperature fluctuations Minimal external heat generation eliminates the need for an air conditioned environment Compact footprint for placement on floor or lab benchtop with tank covers minimizing water evaporation Optimized protocols for both plates and Array Tape Simple and intuitive user interface for easy use Optional barcode tracking with accompanying software Reports generation and log file reviewer Chemistry Supports all common end-point PCR chemistries and protocols as well as emulsion PCR (ePCR) Provides uniform temperature across the entire plate or array for consistent reactions and accurate data

Araya® In-Line Fluorescence Detection System Fully Automated Fluorescence Detection System Araya® Fluorescent Scanner Araya is an inline fluorescence detection instrument that includes fully automated Array Tape® in-feed, bar code reading, fluorescence detection, and Array Tape out-feed. Detection may be placed at one or more process points within the system configuration, providing a high degree of flexibility in process design. Scan Time for 384-Well Array The typical scan time of a 384-well array in Array Tape is 20 to 30 seconds. PMT-Based Reading Modes The Araya is a PMT-based system supporting detection of three different dyes in a single well. Each dye is read in a single back and forth pass over a column in the Array Tape. Current customers have selected mirrors and filters to read TaqMan™ and InvaderPLUS™ assays utilizing single (FAM, VIC, and ROX) or multiple dyes (FAM with "Redmond" Red). The modular design of the Araya is ideally suited for integration of other scanning technologies. If your application requires different reading modes, Contact Us about a custom solution. Data Logs Output All run information is logged in XML files. The log files can be queried for errors, sample location, and dispense parameters while the run is in process. Configurable XML output LIMS summary files are also available. For information on data management and scoring, go to the Intellics® Software overview.

AmbiStore™ Plate Handling and Storage System AmbiStore Plate Storage The AmbiStore plate handling and storage system from HighRes Biosolutions has been integrated into the Nexar. Sample and assay plates are rapidly delivered from the AmbiStore to the Nexar for transfer into Array Tape® wells. The AmbiStore adds significant storage capacity (managing up to 924 low-profile plates in the M model) with integrated barcode scanning for fast inventory tracking.

• Volume Range250 nL to 2,500 nL
• MechanismMicro-solenoid valves
• Channels4 or 8

Multi-Channel Dispense Jet Four Tip Dispense Jet Nexar FlexJet Dispense Jet The Dispense Jet is an independent, multi-channel attachment containing four or eight nozzles to aspirate and dispense reagent from a plate to Array Tape®. The Dispense Jet moves horizontally (x and y-axis) and vertically (z-axis) on the Positioning Rail, allowing the nozzle(s) to reach any well in the Array Tape. Typically, a large volume of reagent is aspirated into each nozzle and smaller volumes are dispensed into the Array Tape. The Dispense Jet Attachment also contains a 1D2D barcode reader that can read the 2D code on the Array Tape and the 1D code on the end of the plate. FOUR TIP DISPENSE JET Mechanism: Single jet solenoid micro-valve Volume Range: 750 nL to 1250 nL Precision: CV Channels: 4 FlexJet® Mechanism: Micro-solenoid valves Volume Range: 250 nL to 2, 500 nL Precision: CV Channels: 4 or 8

• Temperature15°C

Array Tape® Sealer Tape Sealer Module Nexar® Array Tape Sealer The Array Tape Sealer Module applies a seal, securing the samples and assays in Array Tape. It contains two spindles, an upper spindle for unwinding the master roll of cover tape and a lower spindle for rewinding the backer tape. The Array Tape path passes below a rubber roller where the cover tape is applied. The rubber roller is engaged with a toggle handle and is monitored by a photoeye. An inline photoeye indicates when the cover tape is empty. Low Seal Sensor A Convenient and Efficient Solution for Cover Seal Outages Eliminate cover seal monitoring challenges with the Low Seal Sensor. The sensor, available as an upgrade to the Array Tape Seal Module, provides reliable and consistent detection of low cover seal levels, supporting proactive instrument pause states. Walk away with confidence knowing the sensor will bring the instrument to a controlled pause, allowing the lab technician to change the cover seal roll without disruption to, or loss of, assays. Tape Sealer Product Features Fiber optic sensor - measures a reflectivity signal directed at the cover seal roll Rolling tape guide - additional precaution that prevents adhesive from reattaching to the tape guide Functions with current software Customizable sensor threshold - the threshold can be adjusted to control the amount of cover seal remaining upon pause. Walk away with confidence knowing the sensor will bring the instrument to a controlled pause. Tape Sealer With Integrated Chiller The array is chilled down to 15°C within seconds to enable precise incubation and temperature.

Douglas Scientific has retro-fitted select models of centrifuges with the capability to spin Array Tape and force the sample to the bottom of the well. An air knife that blows a steady stream of air through the Array Tape aids in the removal of moisture that may have become trapped between layers during PCR water bath.

Inline Araya Detection Module Nexar® Inline Detection The inline Araya detection module configured into the Nexar® liquid handling and assay processing system allows laboratories to capture data in line with liquid handling and amplification. The Araya is a PMT-based system supporting detection of 3 fluorescent dyes in each reaction well. Inline detection modules are currently utilized for the following applications: End-point Isothermal DNA Amplificiation Real Time Isothermal DNA Amplificiation qPCR Learn more about inline detection with the Araya on the Araya Detection System page.