Raj Biosis Private Limited - Elisa Test

Cancer Antigen 125 (CA125) is a surface antigen associated with epithelial ovarian cancer. In serum, CA125 is associated with a high molecular weight glycoprotein. Published studies have indicated that elevated serum CA125 levels can be found in individuals with serious endometroid, clear-cell and undifferentiated ovarian carcinoma. The serum CA125 concentration is greater than 35 units per ml in 60% of women with ovarian cancer and >80% of patients with disseminated ovarian cancer. The serum CA125 is elevated in 1% of normal healthy women, 3% of normal healthy women with benign ovarian diseases, 6% of patients with non-neoplastic conditions (including but not limited to first trimester pregnancy, menstruation, endometriosis, uterine fibrosis, acute salphingitis, hepatic diseases and inflammation of peritoneum, pericardium or pleura). Serial determinations of serum CA125 as well as pelvic examination increase the test specificity. Serum CA125 concentration may be useful in monitoring treatment and distinguishing between good response to treatment and progressive malignant disease with poor therapeutic response.

Ultra Dengue NS1 Ag ELISA

Ultra Dengue NS1 Ag ELISA is an enzyme linked immunosorbent assay for the detection of Dengue NS1 antigens in human, serum or plasma. This test is intended for professional use as an aid to early diagnosis of Dengue virus infection.This test is for in vitro only.

The B12 test kit is a solid phase enzyme-linked immunoassay (ELISA), based on the principal of delayed competitive binding. Streptavidin coated wells are incubated with extracted Vitamin B12 standards, controls, samples, and Intrinsic Factor-Biotin conjugate at room temperature for 45 minutes. During the incubation, the biotin-labeled intrinsic factor will bind to the Vitamin B12 in the sample, standard, or quality control serum. After the 45 minute incubation, Vitamin B12-Enzyme conjugate is added which competes with the Vitamin B12 of the sample, standard, or quality control serum for remaining sites on the Intrinsic Factor for an additional 30 minutes. All unbound conjugates are then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm