ADVANTAGE MAL CARD is a visual, rapid and sensitive immunoassay for the qualitative diagnosis of P.falciparum and other Plasmodium Species (P.vivax/ P.malariae/ P.ovale/ P.falciparum) based on pLDH antigen in human whole blood.
ADVANTAGE MAL CARD is an immunoassay based on the “Sandwich principle” .The conjugate contains colloidal gold conjugated to monoclonal anti-pan specific pLDH (plasmodium lactate dehydrogenase) antibody. The test uses monoclonal anti-Pf pLDH antibody (test line F) & monoclonal anti-Pan specific pLDH antibody (test line P) immobilized on a nitrocellulose strip. The test sample is added to the device. On addition of assay buffer, the red blood cells get lysed. If the sample contains P.falciparum or P.vivax/P.malariae/P.ovale, the colloidal gold conjugate complexes the P.f specific pLDH/Pan specific pLDH in the lysed sample. This complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody, the complex is trapped forming a purplish pink band which confirms a reactive test result. Absence of a coloured band in the test region indicates a negative test result. To serve as a procedural control an additional line of anti-mouse antibody has been immobilized on the strip as control.