Efficiency of Transfer Hi-fidelity of transfer as large open surface areas in cassette frame allows unhindered passage of currentShort transfer times as low resistance to passage of current due to cassette frame design and sponge quality Economical Less requirement of bufferShort run times as better efficiency of transfer currentNo cold room required as it can be run in the refrigerator due to its small foot printSaves reagent and operator cost as leakage and errors are prevented