Botulinus TypeA B PCR Diagnostic Kit

Reagen™the botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type a/b) in the feces, water and serum sample.but the result is only advisory.
 procedure overview
reagen™the botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) a/b type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) a/b dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) a/b.
 kit contents, storage and shelf life
kit contents amount storage
nucleic acid extraction buffer 2ml -20°c
taq dna polymerase 50μl -20°c
bl-a/b-qpcr mix 1ml -20°c
bl-a/b -positive control 50μl -20°c
negative control 250μl -20°c
the kit’s specifications is 48 reactions/box,the validity of this kit is six months , should be preserved in - 20 ℃, avoid repeated freezing and thawing.
 required materials
pcr instrument with double or more channels.
 sample collection and storage and transportation
1. Human or animal feces
collect fresh feces with mucus purulent blood, sealed samples, then test.
2. Blood
collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples,then test.
 dna extraction