CDNA Synthesis Kit

Other Details :

• Thermo Scientific Verso CDNA synthesis Kit provides robust transcription of RNA to create a complete CDNA pool
• The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system
• The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure
• The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the CDNA synthesis buffer has been optimized to achieve a full and diverse CDNA pool

Highlights :

• Highly sensitive with a broad dynamic range: can reverse transcribe RNA from an input range of 1pg to 1µg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42^oC to 57^oC) for success with GC-rich and other difficult templates
• Included RT Enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase Inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR

Applications :

• 2-step RT-(q) PCR
• Single stranded CDNA synthesis

Features :

• Moisture proof packing
• Safe to use
• Accurate composition