HCP Antibody Development Service

Ideally, any technology for detecting Host Cell Protein (HCP) should: (i) detect protein concentrations across a wide dynamic range, from extremely low concentrations of individual impurity proteins to much higher concentrations of impurity proteins; (ii) track changes in HCP populations and concentrations throughout the entire bioprocess; (iii) simultaneously monitor and measure multiple protein analytes; and (iv) monitor and measure low concentration HCP even in the presence of target recombinant protein at high concentrations. Additionally, if a risk-based assessment of HCP is necessary, these methods should be capable of identifying HCP at any stage of the bioprocess. Employing anti-HCP antibodies for qualitative and quantitative detection and removal of HCPs is highly effective. Thus, the development of anti-HCP antibodies is of significant importance.

1. Identification of HCP Antigens

A variety of cell lines, including mammalian, insect, plant, and prokaryotic cells such as Escherichia coli, are employed for producing recombinant proteins for therapeutic purposes. Chinese hamster ovary cells (CHO) are widely used in the biotechnology industry. For instance, pIs range from ~3-11, with molecular weights from ~5kDa to ~250kDa for large proteins. During the purification process, the content of the product protein gradually increases, while the content of host impurity proteins significantly decreases, dropping from >10-90% to...

About Us

MtoZ Biolabs is an integrated Contract Research Organization (CRO) dedicated to providing cutting-edge chromatography and mass spectrometry services for researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our team of experts and advanced technological platforms are designed to support your research projects with precision and reliability.

Email: marketing@mtoz-biolabs.com