Label-Free Quantitative Proteomics Service, MS Based

Label-free analysis is a powerful technique for identifying and quantifying relative changes in complex protein samples. In this method, samples are run individually and identified by MS/MS information. Relative quantitation of different samples is then analyzed by aligning chromatographic peak areas of precursor ions between various runs. The most distinctive advantage is that it quantifies proteins without any use of labels, and can analyze unlimited number of samples. Since Label-free is relatively simple and easy compared to other proteomics techniques, it has been widely used for proteomics study and biomarker discovery. MtoZ Biolabs is proud to offer Label-free quantitative proteomics service with faster simpler results. We can also perform MS3 analysis to ensure higher analytical accuracy of qualification and quantitation.MtoZ Biolabs provides integrate Label-Free Quantitative Proteomics Service, MS Based.

Analysis Workflow

Isotopic Labels are Not Required|Simpler Experimental Procedures and Data Analysis|Samples are Run Individually, and Numbers of Samples are Unlimited|Suitable for Analyzing Various Types of Samples, Including Tissue, Cell, Blood, etc.

Sample Submission Requirements

*Note: For Co-IP protein samples, only 2-5 ug is required. During sample elution, detergents (such as SDS) and high oxygen concentration should be avoided. Alternatively, you can send the sample beads to us directly.

Bioinformatics Analysis

Label-Free MS Data Quality Assessment|Multivariate PCA Analysis|Protein Statistical Analysis: Venn Diagram, Volcano Plot|Functional Annotation: GO Annotation, KEGG Annotation, COG Annotation|Clustering Analysis: Hierarchical Clustering, K-Means Clustering|Network Analysis: STRING Analysis

Deliverables

Experiment Procedures|Parameters of Liquid Chromatography and Mass Spectrometer|MS Raw Data Files|Peptide Identifications and Intensities|Protein Identifications and Intensities|Bioinformatics Analysis

Related Services

Quantitative Proteomics

Label-Free

iTRAQ/TMT

SILAC/Dimethyl

SWATH

MRM

Protein Analysis

Protein Identification

Protein Mass Measurement

PTMs Identification

PTM Analysis

Phospho-Proteomics

Acetyl-Proteomics

Ubiquitin-Proteomics

Glyco-Proteomics

Disulfide Bond

Histone Modifications