Features

One-Step RT-PCR Kit is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.

Advantages & Features :

• Fast and easy one-tube setup
• Efficient one-step RT-PCR of any RNA template without optimization
• Unique enzyme mix for high specificity and sensitivity
• Balanced mixture of enzymes with optimized reverse-transcription buffer 

Applications : 

For use in all common Molecular Biology applications, such as :

• PCR.
• Real-time PCR.
• High fidelity and long PCR.
• RT-PCR.
• cDNA synthesis.
• DNA sequencing.
• DNA amplification

Features

One-Step RT-PCR Kit is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.

Advantages & Features :

• Fast and easy one-tube setup
• Efficient one-step RT-PCR of any RNA template without optimization
• Unique enzyme mix for high specificity and sensitivity
• Balanced mixture of enzymes with optimized reverse-transcription buffer 

Applications : 

For use in all common Molecular Biology applications, such as :

• PCR.
• Real-time PCR.
• High fidelity and long PCR.
• RT-PCR.
• cDNA synthesis.
• DNA sequencing.
• DNA amplification