Platelet Diluting Fluid

Features

• Widely used in hospitals, nursing homes and clinics
• Prepared using premium ingredients
• Longer shelf life
• Pure compositions

Information

• Reagents supplied: Cat. No. HE 892
• Platelet diluting fluid 100ml

Composition

• Tri sodium citrate: 3%
• Na CI: 1%
• Formaldehyde: 1%
• Detergent: 1%

Preparation of Working Reagent

• The reagent is ready to use
• Filter through whatmai no.1, if necessary

Storage & Stability

• Stable at 25-35 degree celcius
• Expiry date printed on the label

Sample

• Whole blood collected in EDTA
• Direct peripheral blood from a finger prick is preferred

Procedure

• Freshly filtered
• Platelet diluting fluid: 2.00ml
• Whole blood (well mixed): 0.02 ml
• Mix well takes 10 minutes to complete fixation and haemolysis
• Mix well and charge one drop in a counting chamber or feed directly to an automatic cell counter
• Keep the chamber for 3 minutes to settle the cells under a petridish moisted with wet cotton gauze to prevent drying
• Platelets can be seen as minute cells retractile in low light under high power objective of a microscope
• Count square millimeter (4WBC column) and calculate platelets per µL
• Expected value: 200,000 to 400,000/µL