Real Time PCR System

The most Powerful DNA amplification technology known as on date In molecular biology first described by (Higuchi, 1992; Higuchi, 1993). Real-Time Polymerase Chain Reaction, also called quantitative Real Time Polymerase Chain Reaction (qPCR) or kinetic polymerase chain reaction, is a laboratory technique based on the polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of a specific sequence in a DNA sampl