Staining Hematoxylin

Hematoxylin Detailed introduction: Synonyms: hematoxylin;h(a)ematineMolecular Formula: C16H14O6Molecular Weight: 302.28Cas No.: 517-28-2 Melting Point:200°C  Hematoxylin-eosin staining (hematoxylin-eosin staining), referred to as HE staining, paraffin section technology commonly used in one of the dyeing method. Hematoxylin is alkaline, mainly in the nucleus of the chromatin and the cytoplasm of the ribosomes purple blue; red for the acid dye, the main cytoplasm and extracellular matrix in the composition of red. HE staining is the most basic and most widely used technique in histology, embryology, pathology teaching and research.  Dye classification:The properties that are easy to be colored by alkaline or acidic dyes are called basophilia and acidophilia, while neutrophils and acid dyes have weak affinity as neutrophilia.The proteins in the tissue constitute a lot of amino acids in the protein, and they have different isoelectric points. In the ordinary dyeing method, the pH value of the dyeing liquid is about pH6, the intracellular acidic substances such as the chromatin of the nucleus, the rough.