Trenbolone ELISA Test Kit

REAGEN™Trenbolone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trenbolone in fish,shrimp, meat and urine.
The unique features of the kit are:
 High recovery (75-105%),rapid(10-40 minutes),and cost-effective extraction methods.
 High sensitivity(0.05 ppb)and low detection limit(0.025ppb) for meat sample.
 High reproducibility.
 A quick ELISA assay(less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The Trenbolone antibody has been coated in the plate wells. During the analysis, sample is added along with the enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the HRP from binding to the antibody attached to the well.The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™Trenbolone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Trenbolone Coated Plate 1 x 96-well Plate(8 wells x 12 strips) 2-8°C
Trenbolone Standards:
Negative control (white cap tube)
0.05 ngmL (yellow cap tube)
0.1 ngmL (orange cap tube)
0.25 ngmL (pink cap tube)
0.5 ngmL (purple cap tube)
1.0 ngmL (blue cap tube)
10 µgmL (spiking, optional, red cap tube)
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
1.0 mL
0.8 mL
2-8°C *
180X HRP Conjugate 180 µL 2-8°C
Sample Diluent buffer 20 mLX2
20X Wash Solution 30 mL
Stop Buffer 14 mL
TMB Substrate 10 mL
Sample Balance Buffer A 10 mL
Sample Balance Buffer B 62g
Tissue Clean Up mix 25g
* If you are not planning to use the kit for over 1 month, store Trenbolone Standards, Trenbolone Antibody and 180X HRP Conjugate at -20°C or in a freezer.
Sensitivity (Detection Limit)
Sample Type Detection Limit (gg or ppb)
MeatFishshrimp 0.025
Urine 0.25
Specificity (Cross-Reactivity)
Analytes Cross-Reactivity (%)
Trenbolone 100
Nandrolone 41
Boldenone 3.4
Testosterone 3.1
Mesterolone 1.4
Equilin 0.8
Estradiol 0.6
Epitestosterone 0.5
Methyltestoster 0.05
Stanozolol 0.03
Pregnenolone 0.03
Required Materials Not Provided With the Kit
 Microtiter plate reader (450 nm)
 Incubator
 Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
 Rotary evaporator or nitrogen gas
 Vortex mixer (e.g. Genie Vortex mixer from VWR)
 10, 20, 100 and 1000 µL pipettes
 Multi-channel pipette: 50-300 µL (Optional)