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dntps set contains 40.4ml of datp, dctp, dgtp and dttp (monosodium salts) at a concentration of 100mm each in sterile deionized water at ph7.5, whose purity is up to 99.5% (hplc). It is free of rnase and dnase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as pcr, dna sequencing, cdna synthesis and nick translation.
Hi Fidelity polymerase is a mixture of thermostable enzymes. It is specifically developed to synthesize length of PCR product up to 25 kb and with low error rate. Hi Fi polymerase synthesizes higher yields of product from genomic DNA, cDNA, and bacterial cultures. It has 2.5 hours half life at 96oC and easily amplify PCR product of G-C rich or secondary structure DNA by adding G- C rich buffer.
The TRAzol RNA Purification Kit provides a simple, reliable, and rapid method for isolating highquality total RNA from a wide variety of samples, including animal and plant cells and tissue, bacteria, and yeast. The kit utilizes the strong lysis capability of TRAzol Reagent. TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample. Total RNA isolated by TRAzol Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.
