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ultra-pure taq dna polymerase (10x pcr buffer is premixed with mgcl2) is a thermostable enzyme which is purified to reduce levels of contaminating dna, making it well suited for pcr and sensitive experiments using bacterial templates or random primers. The high purity of this taq dna polymerase makes it ideal in detecting and identifying bacterial dna, and is a more accurate method for mutation scanning techniques while preventing the amplification of undesired dna sequences. Ultra-pure taq dna polymerase is suitable for work in bacterial genomics due to the reduced probability of contamination leading to non-specific amplification or artifacts during pcr reactions. The amplified products are up to 8 kb with 3’ adenosine residues and are ready to use directly in ta cloning.
dNTP DNA sequencing kit is a ready-to- use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of10 mM each in sterile deionized water at pH7.5, whose purity is ≥99% (HPLC). It is free of RNase and DNase, and issuitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNAsynthesis and nick translation.
Description
Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts.
Unit description
One unit is defined as the amount of enzyme that will incorporate 10n mole of dNTP into acid-insoluble material in 30 minutes at 74oC.
