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Palghar, Maharashtra, India
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Pigments #2193712

Wright Stain

  • C.A.S. No. 68988-92-1
  • Solubility 0.1 % (MeOH) Clear solution
  • Absorption Maximum (MeOH) ?1max 640-645nm
  • Absorption maximum (MeOH) ?2max 522-525nm
  • Absorptivity (A1%/1cm in MeOH at ?1max) >1100
  • Absorptivity (A1%/1cm in MeOH at ?2max) >650
  • Loss on drying (110°C) <10%

Chemical Name

[7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;2-(2, 4, 5, 7-tetrabromo-3, 6-dihydroxyxanthen-10-ium-9-yl)benzoic acid

 

InChI Key

AXIKDPDWFVPGOD-UHFFFAOYSA-O

 

Solubility

In Methanol 10mg/10 mL, soluble in Ethanol.

 

Wright stain is a commonly used hematological stain. This stain is used in blood smear preparations and has also been described in the staining of microorganisms.

 

Applications:

It is used primarily to stain peripheral blood smears and bone marrow aspirates which are examined under a light microscope. Romanowsky stains are used to perform differential white blood cell counts and to study red blood cell morphology. Abnormal granulocyte, lymphocyte or monocyte cell counts may be used to facilitate the diagnosis of diseases such as leukemia or bacterial infections.

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Alkali Blue

  • C.A.S. No. 1324-80-7
  • CI NO. 42765
  • M.W. 573.65 gm/mole
  • Solubility 0.1% ( Water) Clear solution
  • Absorption Maximum(Methanol) ? max. 593-613nm
  • Absorptivity (MeOH +1N 1ml HCl) >950
  • Dye content (by spectrophotometry) 50%
  • Loss on drying (110°C) <5%
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Indoine Blue

  • Appearance Brownish to black powder
  • C.A.S. No. 4569-88-4
  • CI NO. 12210
  • M.F. C30H24ClN5O
  • M.W. 506.01gm/mole
  • Solubility 0.1%(Methanol) Almost clear solution
  • Absorption Maximum(in MeOH) ?1max 595 - 601 nm
  • Absorptivity 1%/ 1cm,in Methanol at ? max. 596.0nm > 690
  • Loss On Drying at 110oC(1hr) < 5%
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Nile Blue Sulphate

  • C.A.S. No. 3625-57-8
  • CI NO. 51180
  • M.F. C40H40N6O6S
  • M.W. 732.84gm/mole
  • Dye Content (by Spectrophotometry) >90%
  • Solubility 0.1% (50% EtOH) Clear solution
  • Absorption maximum (50% EtOH) ?max 633-639nm
  • Absorptivity (A1%/1cm in 50% EtOH, at ?max) >1630
  • Loss On Drying (110°C) <8%

Chemical Name

Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) sulphate

 

InChI Key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

 

Solubility

soluble in water and ethyl alcohol

 

Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol.Nile Blue A is used to differentiate melanins and lipofuscins. Also useful for staining fats. Used in the preparation of an amperometric glucose sensor.

 

Applications:

Nile blue A is a satisfactory stain for PHB granules in bacteria. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures[1]. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein[2]. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule[3].

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