Double Beam UV Visible Spectrophotometer With PC Controlled Software

Basic Mode

To measure the Absorbance and transmittance

Quantitative

• Coefficient Method
• Standard Curve Up to 10 Standard sample may be used to establish a curve. Four methods for fitting a curve through the calibration points : Linear fit. Linear fit through zero, Square fit and cubic fit

DNA/Protein Test

Concentration and DNA purity are quickly and easily calculated: Absorbance rations: 260 nm / 280 nm with optional subtracted absorbance at 320 nm. DNA concentration = 62.9XA260-36.0XA280 Protein concentration = 1552xA260-757.3xA 280

Wavelength Scan

• The wavelength scan intervals are 0.1,0.2,0.5,1,2,5 nm
• High, Medium and low scan speed are available. They vary from 100 to 3600 nm/min
• Wavelength are scanned from high to low so that the instrument waits at high WL. And it minimizes the degradation of UV sensitive samples.

Kinetics

Abs vs time graphs is displayed on the screen in real-time wait time and measurement time up to 12 hours may be entered with time interval of 0.5,1,2,5,10,30 seconds and one min. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for rate calculation. Rate is calculated using a linear regression algorithm before multiplying be the entered factor.